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Detection of Babesiosis
BabeSIOSIS.net
Babesiosis or piroplasmosis is a very dangerous and invasive zoonotic disease (transmitted between animals and humans) of humans and animals that is spread by ticks. Although this parasite is widely widespread, it commonly infects rodents, carnivores, and cattle. However, in recent years, their spread and cross-spread have increased significantly and has been detected in various species. The causative agent is the Babesia species.
Currently the best way to identify is through good molecular biology techniques such as immunological and serological methods of high specificity and sensitivity such as Polymerase chain reaction (semi and quantitative). The method involves designing primers against the beta-tubuline protein of the piroplasmid parasite Babesia from infected blood cells for diction of messenger RNA. Though detection of this gene is not always easy and the lack of a marker remains is a problem. In recent years new rapid methods for tick-borne parasites have been introduced for detection with greater sensitivity such as the complement fixation test, indirect fluorescent antibody test, loop-mediated amplification, reverse line blotting, Nucleic Acid Sequence Based Amplification and enzyme linked immunosorbent assay.
This advancement in molecular techniques and development of further tools will be useful in the future to identify genes and markers involved in the fight against this ever increasing disease.
This advancement in molecular techniques and development of further tools will be useful in the future to identify genes and markers involved in the fight against this ever increasing disease.
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